General Cell Based Assays
American Research Products Inc TFE3 Transcription Factor Activity Assay
TFE3 Transcription Factor Activity Assay
Eurofins Discoverx PathHunter eXpress IL1RL1/IL1RAP Dimerization Assay, 2pl Kit. (93-1067E3CP5M)
The PathHunter IL1RL1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-33) induced dimerization of IL1RL1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RL1 target protein fused to PK alongside IL1RAP target protein fused to EA. Upon ligand-induced dimerization of IL1RL1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human BMP-4 (BMPR1A/BMPR2) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human BMP-4 (BMPR1A/BMPR2) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of BMPR1A/BMPR2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress OPRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0213E3CP0M)
The PathHunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-2. Activation of OPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human Activin B (ACVR1C/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Activin B (ACVR1C/ACVR2B) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of ACVR1C/ACVR2B. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Epoetin Alfa Bioassay Kit, 2-Plate. (93-0965Y3-00017)
The PathHunter Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or epoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Human IL-23 (IL23R/IL12RB1) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-23 (IL23R/IL12RB1) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL23R/IL12RB1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress GPR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0634E2CP1L)
The PathHunter GPR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR6 and EA-tagged β-Arrestin-2. Activation of GPR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Mouse Pentraxin 3 ASY
The Meso Scale Discovery R-PLEX Mouse Pentraxin 3 Assay is a multiplex immunoassay that quantitatively measures mouse Pentraxin 3 in serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The assay requires 50 L or less sample volume and can perform up to 80 tests across two plates The assay has a lower limit of detection of 0 04 ng/mL and an upper limit of 10 000 ng/mL This assay allows for the rapid reproducible quantification of mouse Pentraxin 3 from various biological matrices
Eurofins Discoverx PathHunter eXpress TGFBR1/TGFBR2 Dimerization Assay, 10-plate Kit. (93-0889E3CP5L)
The PathHunter TGFBR1/TGFBR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/TGFBR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Red Upward cADDis cAMP sensor Big Sky BacMam 5mL
Detect cAMP signaling kinetics in living cells with the Red Upward cADDis fluorescent cAMP assay. Increase in fluorescence indicates increase in cAMP. Packaged in Big Sky BacMam, for enhanced expression in HEKs, CHOs, and many other mammalian cell types and iPSCs. This product is an updated version of U0200R, with improved expression efficiency from Big Sky BacMam.
Eurofins Discoverx PathHunter Human PPAR gamma Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPAR gamma Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated PPARγ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADRA2A β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADRA2A β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADRA2A (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCR7 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 10-Plate Kit. (93-0875E3CP16L)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.